Journal: Frontiers in Pharmacology
Article Title: The type I ribosome-inactivating protein α-MMC induced significant apoptosis of lung cancer A549 and 95-D cells by activating the caspase cascade through TNF signaling pathway
doi: 10.3389/fphar.2025.1529151
Figure Lengend Snippet: Effect of α-MMC on mitochondrial membrane potential in A549 and 95-D cells. α-MMC interfered with A549 cells for 72 h and 95-D cells for 48 h, respectively. (A) Cell ROS levels were measured by flow cytometry. (B) The changes of MMP after JC-1 staining were observed by fluorescence microscopy. (C,D) MMP was analyzed by flow cytometry and displayed in the histogram.
Article Snippet: The effect of α-MMC on morphological changes of A549 and 95-D cells was analyzed by phase contrast microscopy (OLYMPUS BX63, shanghai, China).
Techniques: Membrane, Flow Cytometry, Staining, Fluorescence, Microscopy